CSHL Single Cell Analyses 2025 Meeting

High-Resolution Insights from Single-Cell and Spatial Transcriptomics with PacBio HiFi Sequencing

Date: Thursday, November 13, 2025
Time: After the morning session (roughly 12:30 pm EDT)
Location: Plimpton Conference Room / Beckman Buliding

Lunch will be provided

PacBio HiFi sequencing delivers long, highly accurate reads that capture full-length transcripts while ensuring precise UMI and barcode detection—eliminating the need for error correction or orthogonal sequencing data. To further enhance sequencing depth, PacBio’s Kinnex kits enable concatenation of bulk, single-cell, or spatial cDNAs into longer fragments, increasing cDNA yield by 8-, 12-, or 16-fold without compromising the read quality required for confident barcode identification and variant calling. This workshop will highlight recent advances in single-cell and spatial transcriptomics using Kinnex technology, showcasing new biological insights in cancer and neuroscience research.

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Area of focus

Application(s) of interest Whole genome sequencingTargeted sequencingRNA sequencingViral sequencingDNA extractionMetagenomics + 16sEpigenetics

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Speakers

Bogdan Sieriebriennikov
Field Applications Support
PacBio

Introductory Remarks

Beatrice Zhang
Reserach Associate
Memorial Sloan Kettering Cancer Center

“Intronic retention of transposable elements in CD8+ T cell exhaustion"

Lieke Michielsen, PhD
Postdoctoral Associate
Weill Cornell Medicine

“Spatial isoform sequencing at single-cell resolution reveals novel patterns of spatial isoform variability in brain cell types”

Sofia Lavista Llanos
Director Process Development
ArgenTag

“Streamlined Instrument-Free Long-Read Single-Cell Platform”

Democratizing Genome Assembly and Annotation

Ted Kalbfleisch, Ph.D., Associate Professor, University of Kentucky

Molecular Genetic Applications Enabled by Platinum Quality Reference Genome Assemblies in Octoploid Strawberry

Mitchell Feldmann, Ph.D., Postdoctoral Fellow, University of California, Davis.