PAG 2023

January 13–18, 2023
San Diego, California

We look forward to seeing you at PAG 2023! Join us to find out how new technology from PacBio can help fuel discovery in plant + animal research.

Complete the form on this page to confirm your attendance at our workshop, request a meeting with us at PAG, or to receive a recording of our talks, posters, and other content we’ll present at the conference.

Not able to attend? Not to worry! Sign up anyway for exclusive, on demand access to our scientific program.

In the meantime, see below and refer back to this page for more on our workshop, talks, and other details about PacBio at PAG 2023.

Highlights of the event include:
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  • here
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WORKSHOP

Actionable genomics at scale

Monday, January 16, 2023 12:50 - 3:00 PM PST
Town and Country Hotel, San Diego, CA

Speakers

Jonas Korlach, PhD
CSO, PacBio
Introductory remarks

Gina Zastrow-Hayes
Genomics Technology Manager,
Corteva Agriscience
Ultra-high throughput workflows for agrigenomic applications

Brad Shaffer, PhD
Professor, UCLA, Director, California Conservation Genomics Project
Conserving California’s biodiversity with genomics

Daniel Portik, PhD
Senior Bioinformatics Scientist, PacBio
More and better: a new era for metagenome assembly

Sarah Carey, PhD
Post-Doctoral Fellow, HudsonAlpha Institute for Biotechnology
Exploring the multiple evolutions of sex chromosomes in plants

Pacbio Poster Presentations

Scalable PacBio HiFi Library Prep for High-Quality Genome Assemblies

Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao  

Jacob Brandenburg, Senior Specialist, Segment Marketing, PacBio

Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes. While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly. We describe a new workflow using the PacBio SMRTbell Prep Kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workflows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity. 

An Evaluation of Bioinformatics Tools for HiFi Plant Pangenomes

Authors: Greg Concepcion, Michelle Vierra 

Greg Concepcion, Staff Engineer, Bioinformatics, PacBio

With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder  pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.

Pacbio Presentations

Monday, October XX X:00 pm PST

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Pacbio colab talk

Monday, October XX X:00 pm PST

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POSTERS

All poster presentations are scheduled for Monday, January 16. Specific times are listed below.
# Title First Author Time
PE0048 Ultra-high throughput multi-omic analysis for agrigenomics on PacBio Revio J. Burke 10:00–11:30 AM
PE0684 Maximizing MAGs from long-read metagenomic assemblies: a new post-assembly pipeline with circular-aware binning J. Wilkinson 10:00–11:30 AM
PO0049 With Nanobind HMW DNA extraction, PacBio provides a complete end-to-end workflow for high quality genomes from a diversity of samples M. Kim 3:00–4:30 PM
PO0063 Isoform resolution for single-cell transcriptomics with highly accurate long-read sequencing G. Young 3:00–4:30 PM
# Title First Author Date
1062 A high-resolution panel for uncovering repeat expansions that cause ataxias Y. Tsai Febraury 28, 2022

VISIT US AT BOOTH #111

Stop by our booth to chat with PacBio staff, get all your questions answered, and demo our new Revio and Onso sequencing systems live!

Show hours:

  • Sunday, January 15: 3:00–8:00 PM

  • Monday, January 16: 9:30 AM–5:00 PM

  • Tuesday, January 17: 9:30 AM–3:00 PM

PacBio Service Providers

Interested in getting a sequencing project started? Stop by our service providers at PAG to talk about project design and next steps.

Booth 211

Booth 406

Booth 503

Booth 523

Booth 319

Booth 434

Follow along @pacbio and #PAG30 on Twitter for exciting event updates!