AGBT Ag 2023
March 27–29, 2023
Hyatt Regency Hill Country, San Antonio, Texas
We look forward to seeing you at AGBT Ag 2023! Join us to find out how new technology from PacBio can help fuel discovery in agrigenomics research.
Complete the form on this page to request a meeting with us at AGBT Ag. To thank you for speaking with us, you will receive a special ecofriendly gift when you attend your scheduled meeting.
In the meantime, see below and refer back to this page for more on our workshop, poster presentations, and other details about PacBio at AGBT Ag 2023.
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Long read sequencing at scale with PacBio's new Revio system. Come learn about ultra-high throughput, sample-to-answer solutions for agrigenomics built around the Revio system and HiFi reads from PacBio. This solution includes automated workflows for whole genome sequencing, complete with epigenetics, as well as high throughput genome annotation with our new MAS-seq kits for RNA solutions.
Tuesday, March 28, 2023, 3:20 - 3:32 PM
Independence Ballroom 1-5
Jacob Brandenburg
Market Development Lead - Plant and Animal Sciences
PacBio
Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao
Jacob Brandenburg, Senior Specialist, Segment Marketing, PacBio
Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes. While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly. We describe a new workflow using the PacBio SMRTbell Prep Kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workflows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity.
Authors: Greg Concepcion, Michelle Vierra
Greg Concepcion, Staff Engineer, Bioinformatics, PacBio
With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.
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Monday, October XX X:00 pm PST
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| # | Title | First Author |
| 613 | Ultra-high throughput multiomic analysis for agrigenomics on PacBio Revio system | J. Burke |
Stop by our booth to chat with PacBio staff and get all your questions answered.
Also be sure to drop by our suite to demo our new Revio and Onso sequencing systems live!
Booth and Suite hours:
