Understanding clonal evolution using game theory and single-cell long-read isoform analysis

Understanding mechanisms that drive cellular differentiation has broad implications for a variety of fields including stem cell research, cancer research, and developmental biology.

Single-cell isoform sequencing with the new PacBio MAS-Seq for 10x Single Cell 3’ kit enables researchers to capture cryptic regulatory events that drive these differentiation processes such as alternative splicing (AS), alternative promoter regulation (APR), and alternative polyadenylation (APA), at single-cell resolution.

In our upcoming webinar, Dr. Nathan Salomonis of Cincinnati Children’s Hospital Medical Center will present how he used the MAS-Seq kit and sequencing on the PacBio Revio system to provide new insights into leukemia clonal evolution, through paired single-cell mutation, transcription, splicing, cell-surface, and cell state analysis. Learn how this methodology allowed for the characterization of unique lineages, an important step in single-cell transcriptomics and cancer research.  

Watch the recording to:

  • How PacBio HiFi sequencing enables higher resolution of single-cell transcriptomes compared to other sequencing technologies. 
  • Why isoforms, not genes, are important for single cell research. 
  • How the MAS-Seq kit provides novel insight into disease biology by providing full-length transcript isoform information at the single-cell level.

Be sure to stay with us after the event for a live Q&A session. And, in case you missed it, catch up on part 1 of this webinar series, in which PacBio scientists provide an overview of the MAS-Seq kit and workflow on SMRT Link.

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Nathan Salomonis, PhD
Associate Professor, Division of Biomedical Informatics, Cincinnati Children’s Hospital Medical Center
Elizabeth Tseng, PhD
Associate Director, Product Marketing, PacBio
Host & moderator

Jeremy Wilkinson, PhD
Segment Lead, Microbiome/Metagenomics, PacBio
Host and moderator

Democratizing Genome Assembly and Annotation

Ted Kalbfleisch, Ph.D., Associate Professor, University of Kentucky

Molecular Genetic Applications Enabled by Platinum Quality Reference Genome Assemblies in Octoploid Strawberry

Mitchell Feldmann, Ph.D., Postdoctoral Fellow, University of California, Davis.