Understanding mechanisms that drive cellular differentiation has broad implications for a variety of fields including stem cell research, cancer research, and developmental biology.
Single-cell isoform sequencing with the new PacBio MAS-Seq for 10x Single Cell 3’ kit enables researchers to capture cryptic regulatory events that drive these differentiation processes such as alternative splicing (AS), alternative promoter regulation (APR), and alternative polyadenylation (APA), at single-cell resolution.
In our upcoming webinar, Dr. Nathan Salomonis of Cincinnati Children’s Hospital Medical Center will present how he used the MAS-Seq kit and sequencing on the PacBio Revio system to provide new insights into leukemia clonal evolution, through paired single-cell mutation, transcription, splicing, cell-surface, and cell state analysis. Learn how this methodology allowed for the characterization of unique lineages, an important step in single-cell transcriptomics and hematology oncology research.
Register to hear about this groundbreaking research and more, including:
Be sure to stay with us after the event for a live Q&A session. And, in case you missed it, catch up on part 1 of this webinar series, in which PacBio scientists provide an overview of the MAS-Seq kit and workflow on SMRT Link.
Ted Kalbfleisch, Ph.D., Associate Professor, University of Kentucky
Mitchell Feldmann, Ph.D., Postdoctoral Fellow, University of California, Davis.