China Exclusive 2023

 

MAS-Seq Promotion

HiFi单细胞全长转录本测序方案，中国区客户专属优惠半价促销

——即刻洞见单细胞水平RNA多样化全长转录本信息！

  • 与常规单细胞测序方法相比，通量提高了 16 倍
  • 支持10x Chromium Next GEM Single Cell 3' 试剂盒生成的 cDNA
  • 目标样本为3,000-10,000个细胞文库
  • 准确识别Cell Barcode和UMI序列，获得单细胞水平全场转录本信息
  • 研究细胞类型特异可变剪切，无需其他测序数据补充验证



三代测序单细胞全长转录本，让这一水平的可变剪切、突变、基因融合研究成为可能。

为助力更多科研工作者开启这项研究，PacBio特别申请中国区专属正装试用半价优惠，填写表单，即可参与。

 产品订购货号: 102-659-600 

 产品名称: MAS-Seq for 10X Single Cell 3’ 

 

购买条件：

促销截止日期：2023年06月30日

仅面向初次订购MAS-Seq产品的新客户

每位客户限购一个

仅限中国区客户

获取 MAS-Seq 促销优惠

By registering on this web page, you are consenting and agreeing to collection and use of that information by PacBio in accordance with its Privacy Policy.

Pacbio Poster Presentations

Scalable PacBio HiFi Library Prep for High-Quality Genome Assemblies

Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao  

Jacob Brandenburg, Senior Specialist, Segment Marketing, PacBio

Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes. While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly. We describe a new workflow using the PacBio SMRTbell Prep Kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workflows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity. 

An Evaluation of Bioinformatics Tools for HiFi Plant Pangenomes

Authors: Greg Concepcion, Michelle Vierra 

Greg Concepcion, Staff Engineer, Bioinformatics, PacBio

With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder  pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.

Presenter name, title, credentials, Presenter name, title, credentials

Presenter name, title, credentials, Presenter name, title, credentials

Pacbio colab talk

Monday, October XX X:00 pm PST

Awesome and potentially long title of colab talk goest here

Lorem, ipsum dolor sit amet consectetur adipisicing elit. Nulla, aliquid? Totam nobis ad aut. Praesentium eligendi ut, sit illum ex officia, nisi voluptas ea quos dicta non exercitationem earum sed!

Lorem, ipsum dolor sit amet consectetur adipisicing elit. Eveniet quasi corporis, ut consectetur optio aut quas tempore, rerum ipsum vel eaque voluptates magnam a molestiae voluptatibus veniam quos architecto asperiores? Lorem ipsum dolor sit amet consectetur adipisicing elit. A quod deleniti, tempora reprehenderit, vel consequatur odit eveniet distinctio culpa libero ipsam? Ducimus quos amet laboriosam ut perferendis eos similique cum. Lorem ipsum dolor sit amet consectetur, adipisicing elit.

Presenter name, title, credentials, Presenter name, title, credentials

Presenter name, title, credentials, Presenter name, title, credentials