Understanding clonal evolution using game theory and single-cell long-read isoform analysis

Tuesday, April 18, 2023
8:30 - 9:30 am (India) | 11:00 am - 12:00 pm (Singapore) | 12:00 - 1:00 pm (Seoul, Tokyo) | 2:00 - 3:00 pm (Melbourne)

Understanding mechanisms that drive cellular differentiation has broad implications for a variety of fields including stem cell research, cancer research, and developmental biology.

Single-cell isoform sequencing with the new PacBio MAS-Seq for 10x Single Cell 3’ kit enables researchers to capture cryptic regulatory events that drive these differentiation processes such as alternative splicing (AS), alternative promoter regulation (APR), and alternative polyadenylation (APA), at single-cell resolution.

In our upcoming webinar, Dr. Nathan Salomonis of Cincinnati Children’s Hospital Medical Center will present how he used the MAS-Seq kit and sequencing on the PacBio Revio system to provide new insights into leukemia clonal evolution, through paired single-cell mutation, transcription, splicing, cell-surface, and cell state analysis. Learn how this methodology allowed for the characterization of unique lineages, an important step in single-cell transcriptomics and cancer research.

Be sure to stay tuned until the live Q&A session, where Elizabeth Tseng and Minning Chin of PacBio will be ready and available to answer any questions you may have.

Register to hear about this groundbreaking research and more, including:

How PacBio HiFi sequencing enables higher resolution of single-cell transcriptomes compared to other sequencing technologies. 
Why isoforms, not genes, are important for single cell research. 
How the MAS-Seq kit provides novel insight into disease biology by providing full-length transcript isoform information at the single-cell level.

In case you missed it, catch up on part 1 of this webinar series, in which PacBio scientists provide an overview of the MAS-Seq kit and workflow on SMRT Link.

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Speakers

Nathan Salomonis, PhD
Associate Professor, Division of Biomedical Informatics, Cincinnati Children’s Hospital Medical Center

Elizabeth Tseng, PhD
Associate Director, Product Marketing, PacBio
Host & moderator

Minning Chin, MSc

Staff Scientist, FAS, Asia Pacific
PacBio

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Ted Kalbfleisch, Ph.D., Associate Professor, University of Kentucky

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Mitchell Feldmann, Ph.D., Postdoctoral Fellow, University of California, Davis.