PacBio APAC MAS-Seq promotion

Exclusive APAC MAS-Seq promotion

Get your first MAS-Seq kit at 50% off

Now through June 30, 2023

Product Qualifying for this special promotion:

Product part number: 102-659-600
Product name: MAS-Seq for 10X Single Cell 3' kit

To order: Contact your local PacBio channel partner or submit the form on this page to be contacted by a member of our team!

image of MAS-Seq kit box

Why choose MAS-Seq single-cell sequencing?

Achieve 16-fold throughput increase compared to regular single-cell Iso-Seq library!
No short reads required
Move beyond gene counting to get full-length isoform information
Reveal cell type-specific transcription – spliced isoforms, expressed variants, fusion genes – for disease and biological discovery

Terms:

Promotion ends June 30, 2023
Promotion cannot be combined with other promotions/programs
Promotion can be used one time per customer
First time MAS-Seq kit purchases only
Promotion is exclusively for the APAC region only

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Applications (s) Whole genome sequencingTargeted sequencingRNA sequencingViral sequencingDNA extractionMetagenomics + 16sEpigenetics

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Pacbio Poster Presentations

Scalable PacBio HiFi Library Prep for High-Quality Genome Assemblies

Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao

Jacob Brandenburg, Senior Specialist, Segment Marketing, PacBio

Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes. While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly. We describe a new workflow using the PacBio SMRTbell Prep Kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workflows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity.

An Evaluation of Bioinformatics Tools for HiFi Plant Pangenomes

Authors: Greg Concepcion, Michelle Vierra

Greg Concepcion, Staff Engineer, Bioinformatics, PacBio

With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.

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Pacbio colab talk

Monday, October XX X:00 pm PST

Awesome and potentially long title of colab talk goest here

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