SAMPLE

Universal Human Reference RNA (Agilent) + SIRV Isoform Mix E0 (Lexogen)

METHODS

• Library prep Iso-Seq Template Preparation for Sequel Systems (PN 101-070-200)
• Sequencing Sequel System II with "Early Access" binding kit (101-490-800) and chemistry (101-490-900)
• Run time:
• • 4 hrs. pre-extension
• • 15 hrs. run time per SMRT Cell
• Reference hs37d5 (GRCh37 with decoy)
• Analysis SMRTlink 7.0 "IsoSeq With Mapping protocol" with hg38+SIRV combined reference genome

FILES

Users are encouraged to work with the mapped, unique transcripts in GFF and FASTQ format provided in the "PolishedMappedTranscripts" subfolder. The two raw movie files are in "subreads" subfolder. The intermediate data, full-length reads, are available in "FullLengthReads" folder.

  PolishedMappedTranscripts/
                        - out.abundance.txt
                        - out.fastq
                        - out.gff

  FullLengthReads/
                       - flnc.bam

  Subreads/
                       - m64012_181221_231243.subreads.bam
                       - m64012_181221_231243.subreads.bam.pbi
                       - m64012_181222_192540.subreads.bam
                       - m64012_181222_192540.subreads.bam.pbi

Download

Download from China: https://downloads-ap.pacbcloud.com/public/dataset/UHR_IsoSeq/

URLs and md5 checksums are listed in URLs.txt