Library preparation and HiFi sequencing applications for AAV

Gene therapy is experiencing unprecedented growth. Accurate, comprehensive data is crucial for successful vector discovery, design, and characterization. HiFi sequencing offers a unique solution, enabling the sequencing of full-length AAV genomes with extraordinary precision.

Watch the recording of this exciting digital event where Edward Oakeley, PhD, from Novartis will share and explain his experience and the developments of the HiFi AAV genome sequencing method over time and how his group has developed a modified library preparation protocol to maximize the usefulness of PacBio HiFi reads for AAV characterization. The modified library prep protocol includes adding an additional step for the synthesis of a complementary second strand in order to capture a greater variety of AAV molecules in the analysis.

You'll gain a deep understanding of the unique advantages that HiFi sequencing brings to the table, empowering you to make informed decisions that can significantly impact the safety and efficacy of gene therapy products.

Key highlights:

Hear real-world success stories: Inspiring accounts from a biopharmaceutical researcher who has harnessed the power of HiFi sequencing to achieve highly accurate reads for AAV vector discovery, design, and optimization. Be inspired by the success and apply the insights to elevate your own research endeavors.
Stay ahead of the curve: Discover the challenges of AAV sequencing and how using long-read sequencing can address these needs, ensuring you stay ahead in the rapidly evolving gene therapy landscape.
Unveil a powerful solution: Learn about PacBio's cutting-edge AAV solution and analyses for vector discovery, design, and characterization. With HiFi sequencing, you can unleash the true potential of your gene therapy research.

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Speakers

Edward Oakeley, PhD
Associate Director of Genomics
Novartis – Speaker and panelist

Nadia Sellami, PhD
Segment Lead, Gene and Cell Therapy
PacBio – Host and moderator

Liz Tseng, PhD
Associate Director, Product Marketing
PacBio – Q&A Panelist

Democratizing Genome Assembly and Annotation

Ted Kalbfleisch, Ph.D., Associate Professor, University of Kentucky

Molecular Genetic Applications Enabled by Platinum Quality Reference Genome Assemblies in Octoploid Strawberry

Mitchell Feldmann, Ph.D., Postdoctoral Fellow, University of California, Davis.