Achieving more comprehensive variant detection at scale in human genetics research
Higher throughput and lower costs for accurate long-read sequencing are now available on the Revio system with its trusted highly accurate long-read chemistry (HiFi chemistry). HiFi sequencing detects all types of variants with high precision and recall, phases haplotypes, and identifies methylation patterns across the genome.
SciLifeLab in Uppsala, Sweden, has implemented the Revio system in their workflow, opening up new opportunities to a range of sequencing applications, including whole-genome HiFi sequencing for human genomic research.
Adam Ameur, PhD, associate professor at SciLifeLab, presents early results of whole-genome HiFi sequencing in human genomic research, as part of the Genomic Medicine Sweden project. Working on a small cohort they achieved up to 30-fold HiFi coverage from a single SMRT Cell per genome. This study focuses on finding variants – ranging from single nucleotide variants (SNVs) to large structural variants (SVs) and epigenetic signals – using a single technology.
In this webinar, Dr. Ameur discusses:
More comprehensive detection of SNVs, SVs, tandem repeats, and epigenetic signals
Near-complete diploid assemblies generated to find complex, potential disease-causing SV regions
Use of the Revio system in future population-scale and single-cell sequencing research
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Speakers
Adam Ameur, PhD Associate professor
SciLifeLab
Mark Chaisson, PhD Assistant Professor
Computational Biology and Bioinformatics
University of Southern California
Guest speaker
Democratizing Genome Assembly and Annotation
Ted Kalbfleisch, Ph.D., Associate Professor, University of Kentucky
Molecular Genetic Applications Enabled by Platinum Quality Reference Genome Assemblies in Octoploid Strawberry
Mitchell Feldmann, Ph.D., Postdoctoral Fellow, University of California, Davis.