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Monday, January 15 | 12:50 – 3:00 PM PST Town and Country Hotel, Room C
Please join us to learn how researchers are utilizing Kinnex from PacBio to transform their multiomic research. From RNA-Seq to 16S at scale, these applications can now produce HiFi reads at much higher orders of magnitude on PacBio long-read sequencing platforms. Attend this session to hear the latest on how HiFi sequencing applications, on our high-throughput Revio system, are being used for comprehensive characterization of plants, animals, and their surrounding ecosystems.
Josh Clevenger, PhD
Faculty Investigator, HudsonAlpha Institute for Biotechnology
Talk title: Next generation plant breeding: harnessing pangenomics to drive gene discovery and selection with HiFi sequencing
Caroline Howard, PhD
Senior Scientific Manager, Tree of Life Programme - Wellcome Sanger Institute
Talk title: Leveraging long read technology to generate reference level genome assemblies at scale
Ethan Thai
Microbiome Operations and Services Manager, Zymo Research
Talk title: Beyond short reads: mastering microbial analysis with Zymo Research microbiome service
Doreen Ware, PhD
USDA ARS, Adjunct Professor, Cold Spring Harbor Laboratory
Talk title: Long-read sequencing broadens access to genetic and transcript diversity for high-value traits in sorghum
Jonas Korlach, PhD
Co-Founder, PacBio
Introductory remarks and workshop overview
Date/Time: Thursday, November 2 | 10:10 – 10:40 AM EDT
Location: Walter E. Washington Convention Center, Exhibit Hall, CoLab Theater #2
Alexander Hoischen, PhD
Assistant Professor, Radboud University Medical Centre
PacBio HiFi long-read sequencing has the potential to become a single front-line assay for interrogating rare disease cohorts because of its ability to accurately call and phase all classes of variants. An international group of clinical researchers and PacBio have been collaborating to study the efficacy of HiFi sequencing in interrogating the many possible genetic mechanisms that underlie rare diseases. This presentation will provide an overview of the group’s goals and activities and will share recent results, particularly around correctly resolving clinically relevant genes that lie in areas of high sequence homology or otherwise complex regions of the human genome.
Date/Time: Friday, November 3 | 10:10 – 10:40 AM EDT
Location: Walter E. Washington Convention Center, Exhibit Hall, CoLab Theater #2
David Porubsky, PhD
Post-Doctoral Fellow, University of Washington, Eichler Lab
Zev Kronenberg, PhD
Senior Manager, Computational Biology, PacBio
Accurate long-read sequencing characterizes the full spectrum of genetic variation across the genome, but variant calling software is still catching up to the sequencing technologies. We have generated deep Pacific Biosciences (PacBio) high-fidelity (HiFi), ultra-long Oxford Nanopore Technologies (ONT), Strand-seq, and Illumina whole-genome sequencing data to construct near-T2T, phased genome assemblies from primary material obtained from a 4-generation, 28-member CEPH pedigree (1463). We are constructing a more comprehensive and validated catalog of >8 million single-nucleotide variants, indels, short tandem repeats, and structural variants, including a detailed assessment of inversion polymorphisms that associate with disease risk. The use of multiple orthogonal technologies, near-T2T phased-genome assemblies, and a multi-generation family allow us to assess inheritance patterns and to create a “truth set” for all classes of human genetic variation upon which to test and benchmark new technologies.
| # | Title | First Author | Time |
| PO0633 | High throughput HMW DNA animal blood extraction and sequencing on the PacBio Revio system | D. Moine | 3:00-4:30 pm |
| PO0651 | A high throughput, low-cost automated library prep method for PacBio long-read sequencing at scale | G. Young | 3:00-4:30 pm |
| PO0767 | New long-read metagenome assembly methods increase the number of high-quality mags from host-associated microbiomes | D. Portik | 3:00-4:30 pm |
| PE0650 | High-throughput full-length isoform sequencing with long, accurate reads using the new Kinnex kits from PacBio | E. Tseng | 10:00-11:30 am |
| PE0766 | Increasing the throughput of full-length 16S sequencing with Kinnex | J. Wilkinson | 10:00-11:30 am |
Stop by our booth to chat with PacBio staff and have your questions answered. We are excited to learn about your sequencing goals and learn more about your research.
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