Hear from Kinnex users
Gain insights from microbial and plant + animal researchers on how they are using the new Kinnex kits (using MAS-Seq technology/method) to push the boundaries of full-length 16S and RNA.
ON-DEMAND WEBINAR
Increasing the throughput of full-length 16S and RNA-Seq with Kinnex™ kits
Register to join the conversation
Join us on Tuesday, Dec. 12 starting at 8:00 AM PST or Wednesday, Dec. 13 starting at 11:00 AM SGT to hear from distinguished speakers as they unveil the potential of Kinnex kits from PacBio. Stay for the live Q&A to have your personal research questions answered.
WEBINAR SUMMARY
Explore the transformative capabilities of Kinnex kits from PacBio, in reshaping the landscape of microbiome community profiling and RNA-Seq in plant and animal research. This webinar will guide you through the enhanced functionalities of full-length 16S rRNA and RNA-Seq, empowered by the new Kinnex kits (using MAS-Seq technology/method), which facilitate a substantial increase in the number of reads obtained from PacBio long-read sequencing platforms. Delve into detailed customer scientific studies that showcase the practical applications and influential impact of Kinnex kits in contemporary research environments.
Register to watch recording
Presentations
Introduction to Kinnex kits for full-length 16S and RNA-Seq
15-minute talk
Jacob Brandenburg, Senior Specialist, Plant and Animal Genomics, PacBio
Kinnex full-length RNA for Plant and Animal Research: Setting a new standard in high-quality genome annotation
20-minute talk
Jeremy Schmutz, Co-Director, HudsonAlpha Genome Sequencing Center
Beyond short-read: Master microbial analysis with Zymo’s full-length 16S microbiome service
20-minute talk
Ethan Thai, Microbiome Operations and Services Manager, Zymo Research
Study of the Dynamics of Microbiome Diversity in Early-Life
20-minute talk
Chinnu Jerard, PharmD, PhD Scholar, Early Life Microbial Immunology, Telethon Kids Institute, Perth Children’s Hospital
Enabling high throughput full-length RNA and 16S sequencing with Kinnex kits
15-minute talk
Jocelyne Bruand, PhD, Senior Staff Scientist, Bioinformatics, PacBio
Hosts and Moderators
25-minute presenter Q&A
Jeremy Wilkinson, PhD, Senior Staff Specialist, Microbial Genomics, PacBio - Host
Elizabeth Tseng, PhD, Associate Director, Product Marketing, PacBio - Q&A Moderator
Plenary talks
Scalable PacBio HiFi library prep for high-quality genome assemblies
Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao
Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes.
While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly.
We describe a new workgow using the PacBio SMRTbell prep kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workgows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity.
bioinformatics tools for HiFi plant pangenomes
Authors: Greg Concepcion, Michelle Vierra
Greg Concepcion, Staff Engineer, Bioinformatics, PacBio
With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.
bioinformatics tools for HiFi plant pangenomes
Authors: Greg Concepcion, Michelle Vierra
Greg Concepcion, Staff Engineer, Bioinformatics, PacBio
With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.
bioinformatics tools for HiFi plant pangenomes
Authors: Greg Concepcion, Michelle Vierra
Greg Concepcion, Staff Engineer, Bioinformatics, PacBio
With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.
Presentations
Monday, June 12, 2022 14:15–15:45 PST
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Elizabeth Tseng, PhD
Title, credentials, PacBio
Elizabeth Tseng, PhD
Title, credentials, PacBio
Elizabeth Tseng, PhD
Title, credentials, PacBio
Elizabeth Tseng, PhD
Title, credentials, PacBio
Presentations + posters
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Poster presentations
# Title First Author Date/Time 2314 Increased Diagnostic Yield With Long Read Sequencing In Patients With Undiagnosed Neurodevelopmental Disorder S. Jamuar October 26, 3:00 - 4:45 pm PDT 2322 Long-read HiFi genome sequencing reveals a 2.7 kilobase intronic insertion in NR5A1as a cause of 46,XY disorder of sexual development G. Del Gobbo October 26, 3:00 - 4:45 pm PDT 2354 Ultra-low DNA input with long-read sequencing identified complex chromosomal rearrangements involving NIPBL in a Cornelia de Lange syndrome patient N. Jiang October 26, 3:00 - 4:45 pm PDT 2723 Accurate CYP2D6 star (*) allele diplotyping for long-read PacBio HiFi sequencing J. Harting October 26, 3:00 - 4:45 pm PDT 3096 PacBio HiFi sequencing provides highly accurate CpG methylation calls without bisulfite treatment C. Saunders October 26, 3:00 - 4:45 pm PDT 3133 Sequencing By Binding (SBB) enables a lower limit of detection for Tuberculosis resistance genes gyrA and katG C. Wike October 26, 3:00 - 4:45 pm PDT 2269 Clinical long-read genome sequencing: Analytical performance of germline small variant detection using HiFi genome sequencing N. Hammond October 27, 3:00 - 4:45 pm PDT 2954 De novo variant detection with HiFi reads J. Lake October 27, 3:00 - 4:45 pm PDT 3012 High throughput workflow for human whole genome sequencing using PacBio HiFi J. Rocha October 27, 3:00 - 4:45 pm PDT 3132 Sequencing By Binding (SBB) demonstrates superior performance in low-pass whole-human-sequencing applications KY. Chen October 27, 3:00 - 4:45 pm PDT 2938 Closing the gap: Solving complex medically relevant regions of the human genome F. Sedlazeck October 27, 3:00 - 4:45 pm PDT -
In-booth presentations
# Title First Author Date/Time 2314 Increased Diagnostic Yield With Long Read Sequencing In Patients With Undiagnosed Neurodevelopmental Disorder S. Jamuar October 26, 3:00 - 4:45 pm PDT 2322 Long-read HiFi genome sequencing reveals a 2.7 kilobase intronic insertion in NR5A1as a cause of 46,XY disorder of sexual development G. Del Gobbo October 26, 3:00 - 4:45 pm PDT 2354 Ultra-low DNA input with long-read sequencing identified complex chromosomal rearrangements involving NIPBL in a Cornelia de Lange syndrome patient N. Jiang October 26, 3:00 - 4:45 pm PDT 2723 Accurate CYP2D6 star (*) allele diplotyping for long-read PacBio HiFi sequencing J. Harting October 26, 3:00 - 4:45 pm PDT 3096 PacBio HiFi sequencing provides highly accurate CpG methylation calls without bisulfite treatment C. Saunders October 26, 3:00 - 4:45 pm PDT 3133 Sequencing By Binding (SBB) enables a lower limit of detection for Tuberculosis resistance genes gyrA and katG C. Wike October 26, 3:00 - 4:45 pm PDT 2269 Clinical long-read genome sequencing: Analytical performance of germline small variant detection using HiFi genome sequencing N. Hammond October 27, 3:00 - 4:45 pm PDT 2954 De novo variant detection with HiFi reads J. Lake October 27, 3:00 - 4:45 pm PDT 3012 High throughput workflow for human whole genome sequencing using PacBio HiFi J. Rocha October 27, 3:00 - 4:45 pm PDT 3132 Sequencing By Binding (SBB) demonstrates superior performance in low-pass whole-human-sequencing applications KY. Chen October 27, 3:00 - 4:45 pm PDT 2938 Closing the gap: Solving complex medically relevant regions of the human genome F. Sedlazeck October 27, 3:00 - 4:45 pm PDT
Visit us at booth X5-364
Stop by our booth to chat with PacBio staff and have your questions answered. We are excited to learn about your sequencing goals and learn more about your research.
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