Understanding full-length single-cell RNA-seq of immune cells in cardiovascular diseases

Discover how full-length isoform sequencing enables you to robustly and cost effectively identify new isoforms at scale and study isoform dynamics in single-cell populations. The MAS-Seq for 10x Single Cell 3’ kit – and its successor, the Kinnex single-cell RNA kit – concatenate isoforms for highly accurate long-read sequencing (HiFi sequencing). This approach increases throughput and reduces costs by giving you a 16-fold throughput increase compared to un-concatenated libraries, without losing accuracy or full-length isoform information.

Anastasiya Grinko, MSc, applied the MAS-Seq kit to profile the single-cell isoform landscape of monocyte-derived macrophages after myocardial infarction. This approach captured thousands of distinct isoforms, making it possible to see cell-type-specific isoform utilization in monocytes and macrophage subtypes and to unveil temporal dynamics of isoform expression.

Watch the recording to:

Understand the importance of elucidating isoforms in single-cell transcriptomics.
Learn how to analyze MAS-Seq and Kinnex single-cell data with the Single-cell Iso-Seq workflow in SMRT Link.
See how the increased throughput of the MAS-Seq single-cell kit enabled the capture of differentially expressed isoforms across cell types in heart tissue after myocardial infarction.

First name *

Last name *

Organizational/company email *

Organization *

Job role *

Country *

State *

Main field of interest *

Comments

By registering on this web page, you are consenting and agreeing to collection and use of your information by PacBio in accordance with its privacy policy

Speakers

Anastasiya Grinko

PhD Candidate, Helmholz Institute for RNA-based Infection Research

Elizabeth Tseng

Associate Director, Product Marketing, PacBio