2024 pacbio SMRT PITCH

Rockies Edition

PACBIO SMRT PITCH – Rockies Edition IS NOW OPEN

Embark on a sequencing adventure with us!

Unlock the potential of your research with a chance to win up to 50 FREE SMRT Cells (a value of $75,000) of HiFi Sequencing!


We want to hear how PacBio HiFi sequencing will propel your scientific discoveries. In 500 words or less, share your vision and you could be one of 5 finalists chosen to present your groundbreaking work to the PacBio commercial team in February 2025 at the Scottsdale Resort & Spa. This is your moment to shine and showcase the transformative impact of your research!


Our commitment is to support and amplify your scientific journey. Each finalist will exemplify their research in a 5-minute presentation slot on February 12 and in advance personalized guidance from a Genomic Specialist to perfect your pitch. Plus, travel & accommodations from Arizona State University, Northern Arizona University, the University of Arizona, Translational Genomics Research Institute, Brigham Young University, or the University of Utah will be covered!


The grand prize? Up to 50 SMRT Cells of PacBio sequencing for your project, with samples needing to be sequenced within 3 months of the award.


Ready to take your research to the next level? Apply now by completing the brief form on this page with your proposal. Embrace the possibilities with PacBio HiFi and let’s make science happen together! Check out the official rules or reach out with any questions about the SMRT Grant.



Details on how to apply

Simply complete the short form on this page and include your 500-word proposal. You can read the official rules or reach out if you have any questions about this SMRT Grant. The submission window closes Friday, December 20, 2024, at 5:00 PM PDT. 


   

The 2023 AAV STAR grant is open

Apply to win free sequencing and a travel stipend to enhance and promote your AAV-based gene therapy research

Tell us in 100 words or less how PacBio HiFi sequencing in your project will contribute to understanding and developing AAV-based gene therapies for a chance to be one of five winners of sequencing on one SMRT cell 8M and a travel stipend to present your work at a PacBio event.

PacBio offers a complete end-to-end workflow to characterize AAV preparations, with the ability to sequence full-length genomes with high accuracy.


Details on how to apply

We encourage junior academic scientists to apply! Simply complete the short form on this page and include your 100-word proposal. You can read the official rules, or reach out if you have questions about this STAR Grant. The submission window closes Wednesday, November 1, 2023 at 5:00 PM PDT.  

Submit your proposal today!

By registering on this webpage, you are consenting and agreeing to collection and use of your information by PacBio in accordance with our privacy policy.

In-person workshop

PacBio sequencing: See what you’ve been missing

Sunday, June 12, 2023 14:15–15:45 Room M 1+2, level 1

Speakers

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

PacBio sequencing and its applications

Neil Ward, GM and Vice President EMEA, PacBio

Plenary talks

Scalable PacBio HiFi library prep for high-quality genome assemblies

Authors: Jacob Brandenburg, Aaron Wenger, Christine Lambert, Heather Ferrao

Jacob Brandenburg, Senior Specialist, Segment Marketing, PacBio

Long-read whole genome sequencing (WGS) for plants and animals has allowed scientists to tackle the assembly of genomes ranging in size, ploidy, and repeat content. HiFi WGS enables complete, accurate, and contiguous assemblies of even the most complex genomes. As a result, HiFi WGS has been adopted by many biodiversity genomics consortia and agriculture researchers to assemble thousands of genomes.

While HiFi WGS simplifies the bioinformatics of genome assembly, library preparation remains a common challenge, especially for scientists sequencing organisms which yield low quantities of DNA or have very large genomes that require multiple libraries for a single de novo assembly.

We describe a new workgow using the PacBio SMRTbell prep kit 3.0 that is amenable to automation while significantly reducing the time (by 50%), cost, and DNA quantity (by 40%) necessary to prepare a sequencing library. We applied the workflow to rice and fly samples and demonstrate that the assembly quality matches that of the previous, more time-consuming library prep workgows. This new workflow offers a more efficient solution for all WGS applications, regardless of organism size or complexity.

bioinformatics tools for HiFi plant pangenomes

Authors: Greg Concepcion, Michelle Vierra

Greg Concepcion, Staff Engineer, Bioinformatics, PacBio

With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.

bioinformatics tools for HiFi plant pangenomes

Authors: Greg Concepcion, Michelle Vierra

Greg Concepcion, Staff Engineer, Bioinformatics, PacBio

With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.

bioinformatics tools for HiFi plant pangenomes

Authors: Greg Concepcion, Michelle Vierra

Greg Concepcion, Staff Engineer, Bioinformatics, PacBio

With the increasing prevalence of high-quality assembled genomes, access to the pangenomes of species is now an achievable possibility for researchers with all research budgets. However, the tools to visualize, analyze, and extract actionable information from these pangenome datasets are still in their infancy. Using a large plant pangenome dataset, we evaluated the pangenome graph builder pipeline for building a pangenome graph of the species and use these evaluations to provide basic recommendations for how to best manipulate and visualize these pangenomic datasets with other downstream tools including the vg toolkit.

Presentations

Monday, June 12, 2022 14:15–15:45 PST

Awesome and potentially long title of talk goes here

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Elizabeth Tseng, PhD

Title, credentials, PacBio

Elizabeth Tseng, PhD

Title, credentials, PacBio

Elizabeth Tseng, PhD

Title, credentials, PacBio

Elizabeth Tseng, PhD

Title, credentials, PacBio

Presentations + posters

  • Poster presentations

    # Title First Author Date/Time
    2314 Increased Diagnostic Yield With Long Read Sequencing In Patients With Undiagnosed Neurodevelopmental Disorder S. Jamuar October 26, 3:00 - 4:45 pm PDT
    2322 Long-read HiFi genome sequencing reveals a 2.7 kilobase intronic insertion in NR5A1as a cause of 46,XY disorder of sexual development G. Del Gobbo October 26, 3:00 - 4:45 pm PDT
    2354 Ultra-low DNA input with long-read sequencing identified complex chromosomal rearrangements involving NIPBL in a Cornelia de Lange syndrome patient N. Jiang October 26, 3:00 - 4:45 pm PDT
    2723 Accurate CYP2D6 star (*) allele diplotyping for long-read PacBio HiFi sequencing J. Harting October 26, 3:00 - 4:45 pm PDT
    3096 PacBio HiFi sequencing provides highly accurate CpG methylation calls without bisulfite treatment C. Saunders October 26, 3:00 - 4:45 pm PDT
    3133 Sequencing By Binding (SBB) enables a lower limit of detection for Tuberculosis resistance genes gyrA and katG C. Wike October 26, 3:00 - 4:45 pm PDT
    2269 Clinical long-read genome sequencing: Analytical performance of germline small variant detection using HiFi genome sequencing N. Hammond October 27, 3:00 - 4:45 pm PDT
    2954 De novo variant detection with HiFi reads J. Lake October 27, 3:00 - 4:45 pm PDT
    3012 High throughput workflow for human whole genome sequencing using PacBio HiFi J. Rocha October 27, 3:00 - 4:45 pm PDT
    3132 Sequencing By Binding (SBB) demonstrates superior performance in low-pass whole-human-sequencing applications KY. Chen October 27, 3:00 - 4:45 pm PDT
    2938 Closing the gap: Solving complex medically relevant regions of the human genome F. Sedlazeck October 27, 3:00 - 4:45 pm PDT

  • In-booth presentations

    # Title First Author Date/Time
    2314 Increased Diagnostic Yield With Long Read Sequencing In Patients With Undiagnosed Neurodevelopmental Disorder S. Jamuar October 26, 3:00 - 4:45 pm PDT
    2322 Long-read HiFi genome sequencing reveals a 2.7 kilobase intronic insertion in NR5A1as a cause of 46,XY disorder of sexual development G. Del Gobbo October 26, 3:00 - 4:45 pm PDT
    2354 Ultra-low DNA input with long-read sequencing identified complex chromosomal rearrangements involving NIPBL in a Cornelia de Lange syndrome patient N. Jiang October 26, 3:00 - 4:45 pm PDT
    2723 Accurate CYP2D6 star (*) allele diplotyping for long-read PacBio HiFi sequencing J. Harting October 26, 3:00 - 4:45 pm PDT
    3096 PacBio HiFi sequencing provides highly accurate CpG methylation calls without bisulfite treatment C. Saunders October 26, 3:00 - 4:45 pm PDT
    3133 Sequencing By Binding (SBB) enables a lower limit of detection for Tuberculosis resistance genes gyrA and katG C. Wike October 26, 3:00 - 4:45 pm PDT
    2269 Clinical long-read genome sequencing: Analytical performance of germline small variant detection using HiFi genome sequencing N. Hammond October 27, 3:00 - 4:45 pm PDT
    2954 De novo variant detection with HiFi reads J. Lake October 27, 3:00 - 4:45 pm PDT
    3012 High throughput workflow for human whole genome sequencing using PacBio HiFi J. Rocha October 27, 3:00 - 4:45 pm PDT
    3132 Sequencing By Binding (SBB) demonstrates superior performance in low-pass whole-human-sequencing applications KY. Chen October 27, 3:00 - 4:45 pm PDT
    2938 Closing the gap: Solving complex medically relevant regions of the human genome F. Sedlazeck October 27, 3:00 - 4:45 pm PDT

Visit us at booth X5-364

Stop by our booth to chat with PacBio staff and have your questions answered. We are excited to learn about your sequencing goals and learn more about your research.

Thank you to our co-sponsors: Colossal Biosciences, as well as The Vertebrate Genomes Laboratory out of The Rockefeller University for supporting the 2024 PacBio SMRT Conserve Grant.

Colossal is a breakthrough bioscience and genetic engineering company that creates disruptive technologies for extinct species restoration, critically endangered species protection, and the repopulation of critical ecosystems that support the continuation of life on Earth.


The Vertebrate Genomes Laboratory (VGL), directed by Dr. Erich Jarvis, serves as a central laboratory for the VGP. The G10K consortium, an international consortium of tissue curators, biologists, conservationists, genome scientists, computer scientists, outreach educators, and more leads the VGP. Dr. Erich Jarvis is the current chairperson of the G10K consortium. The G10K leadership and community of scientists built an infrastructure from sample collection to genome sequencing, assembly, annotation, alignments, public data releases, and analyses for publications. In addition to the VGL, the genome sequencing hubs also include the Sanger Institute in the UK and the Max Planck Institute in Dresden, Germany.


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