Confidently sequence even the most difficult regions of the genome
PacBio HiFi sequencing and PureTarget technology make it possible to resolve genomic regions that short reads struggle to capture.
Download this technical guide to learn how PureTarget custom panels expand beyond fixed assays, enabling you to resolve difficult loci such as tandem repeat expansions and large non-repeat regions with the accuracy and detail of HiFi long reads.
What you'll learn:
✔ How to create “slim” or in silico panels by removing unwanted targets without changing lab workflows.
✔ Best practices for adding new targets, including repeats, short regions, and long loci requiring multiple guide pairs.
✔ Strategies for benchmarking and validating panel performance using built-in control targets (X-linked UBL4A and autosomal ACTB and GAPDH).
✔ gRNA design strategies and considerations for high on-target performance with minimal off-target risk.
✔ Case studies showing applications in CFTR sequencing, humanized mouse models, and zebrafish mosaicism.