Introduction to amplification-free gene editing characterization using HiFi

Gene editing using CRISPR-Cas9 or similar systems is foundational in many gene therapies. The repair of CRISPR-Cas9 induced double-strand breaks can produce a wide range of outcomes, including small to larger indels of up to several kbs, as well as integration of template constructs at target sites. Accurately characterizing these events is critical in research and drug development.

Short-read sequencing can miss important information such as longer insertions and deletions, or structural variations like chromosomal rearrangements. In addition, commonly used amplification-based methods may introduce unintended biases.

In this webinar, we introduce how highly accurate HiFi long-read sequencing enables a more complete, unbiased view of gene editing outcomes. You’ll learn how amplification-free workflows, such as PureTarget, can capture on- and off-target edits—including larger and more complex events—without the limitations of PCR-based methods.

Watch the recording to to:

Understand the benefit of both high accuracy and read length for gene editing characterization
Discover how amplification-free long-read sequencing can overcome the shortcomings of amplicon-based approaches
Hear how researchers at Uppsala University use HiFi sequencing in their work characterizing gene editing outcomes

Introduction to amplification-free gene editing characterization using HiFi sequencing

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